Effect of total flavones of astragalus on apoptosis and inflammation of alveolar epithelial cells induced by tumor necrosis factor alpha through microrna-221
By: Chen, Xiangyan
.
Contributor(s): Long, Zhihuan.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2023Edition: Vol.85(4), Jul-Aug.Description: 1150-1156p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To explore the effect and molecular mechanism of total flavonoids of Astragalus on the apoptosis and
inflammation of alveolar epithelial cells induced by tumor necrosis factor alpha. Human alveolar
epithelial cells were divided into control group, tumor necrosis factor alpha group, tumor necrosis factor
alpha+trifluoroacetic acid-low, tumor necrosis factor alpha+trifluoroacetic acid-medium, tumor necrosis
factor alpha+trifluoroacetic acid-high group, tumor necrosis factor alpha+anti-microRNA-NC group,
tumor necrosis factor alpha+microRNA-221 inhibitor group, tumor necrosis factor alpha+trifluoroacetic
acid-high+microRNA-NC group, tumor necrosis factor alpha+trifluoroacetic acid-H+microRNA-221
mimic group. Real-time fluorescence quantitative polymerase chain reaction was used to detect the
expression of microRNa-221; (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to
detect the cell proliferation inhibition rate; flow cytometry to detect the apoptosis of alveolar epithelial
cells; enzyme-linked immunosorbent assay to detect the levels of interleukin-1 beta, I interleukin-6
and interleukin-10. In tumor necrosis factor alpha-induced alveolar epithelial cells, the expression of
microRNA-221 was increased, the cell proliferation inhibition rate and apoptosis rate were increased, the
levels of interleukin-1 beta, interleukin-6 were increased and the level of interleukin-10 was decreased
(p<0.05). After treatment with medium and high concentrations of total flavones of Astragalus, the
expression of microRNA-221 in alveolar epithelial cells induced by tumor necrosis factor alpha was
decreased, cell proliferation inhibition rate and apoptosis rate were decreased, interleukin-1 beta
and interleukin-6 levels were decreased and interleukin-10 was increased (p<0.05). Inhibition of
microRNA-221 expression can inhibit tumor necrosis factor alpha induced apoptosis and inflammation
of alveolar epithelial cells. Overexpression of microRNA-221 can reverse the effect of total flavonoids
of Astragalus on apoptosis and inflammation of alveolar epithelial cells induced by tumor necrosis
factor alpha. Total flavones of Astragalus inhibited tumor necrosis factor alpha induced apoptosis and
inflammation of alveolar epithelial cells by down-regulating microRNA-221.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2024-0426 |
To explore the effect and molecular mechanism of total flavonoids of Astragalus on the apoptosis and
inflammation of alveolar epithelial cells induced by tumor necrosis factor alpha. Human alveolar
epithelial cells were divided into control group, tumor necrosis factor alpha group, tumor necrosis factor
alpha+trifluoroacetic acid-low, tumor necrosis factor alpha+trifluoroacetic acid-medium, tumor necrosis
factor alpha+trifluoroacetic acid-high group, tumor necrosis factor alpha+anti-microRNA-NC group,
tumor necrosis factor alpha+microRNA-221 inhibitor group, tumor necrosis factor alpha+trifluoroacetic
acid-high+microRNA-NC group, tumor necrosis factor alpha+trifluoroacetic acid-H+microRNA-221
mimic group. Real-time fluorescence quantitative polymerase chain reaction was used to detect the
expression of microRNa-221; (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to
detect the cell proliferation inhibition rate; flow cytometry to detect the apoptosis of alveolar epithelial
cells; enzyme-linked immunosorbent assay to detect the levels of interleukin-1 beta, I interleukin-6
and interleukin-10. In tumor necrosis factor alpha-induced alveolar epithelial cells, the expression of
microRNA-221 was increased, the cell proliferation inhibition rate and apoptosis rate were increased, the
levels of interleukin-1 beta, interleukin-6 were increased and the level of interleukin-10 was decreased
(p<0.05). After treatment with medium and high concentrations of total flavones of Astragalus, the
expression of microRNA-221 in alveolar epithelial cells induced by tumor necrosis factor alpha was
decreased, cell proliferation inhibition rate and apoptosis rate were decreased, interleukin-1 beta
and interleukin-6 levels were decreased and interleukin-10 was increased (p<0.05). Inhibition of
microRNA-221 expression can inhibit tumor necrosis factor alpha induced apoptosis and inflammation
of alveolar epithelial cells. Overexpression of microRNA-221 can reverse the effect of total flavonoids
of Astragalus on apoptosis and inflammation of alveolar epithelial cells induced by tumor necrosis
factor alpha. Total flavones of Astragalus inhibited tumor necrosis factor alpha induced apoptosis and
inflammation of alveolar epithelial cells by down-regulating microRNA-221.
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